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KMID : 0545120130230030375
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 3 p.375 ~ p.381
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Isolation of a Korean Domestic Microalga, Chlamydomonas reinhardtii KNUA021, and Analysis of Its Biotechnological Potential
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Hong Ji-Won
Jeong Ji-Eun
Kim Sung-Hong
Kim Sung-Hwan
Yoon Ho-Sung
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Abstract
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A freshwater microalga, Chlamydomonas reinhardtii KNUA021, was characterized for its potential as a biochemical feedstock. Its optimal growth was observed when the culture was incubated at 25oC and pH 9.4. However, the isolate was capable of survival and growth under a variety of temperatures (10-30oC) and pH (pH 4.0-12.0) conditions. The total lipid content of the isolate was 21.7% of dry weight and it was found that a high-value fatty alcohol, hexadecenol (C20H40O), was autotrophically produced by strain KNUA021. In addition, a nutritionally important C18:3 ω3 (α-linolenic acid, ALA) was also identified in this photosynthetic microorganism as one of the major fatty acids. Hence, C. reinhardtii KNUA021 appears to show promise for use in the production of microalgae-based biochemicals.
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KEYWORD
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Chlamydomonas reinhardtii, fatty acid, fatty alcohol, microalga
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